Electrophoretic analysis of urinary proteins in domestic animals with acid violet 17 staining.

Electrophoretic Analysis of Plasma and Urinary Proteins.

Accurate analysis of the protein fractions of human plasma has in the past been very difficult and time-consuming. Fractional precipitation by neutral salt, as in Howe's method (1), has proved useful because of its simplicity and because the most important pathologic variations in plasma proteins involve a gross diminution in the albumin fraction. Such methods have definite limitations: Butler'...

P-9: A New Morphology Staining Method for Sperm of Some Domestic Animals

Background: There are some methods for morphology staining of sperm. Although many different and also new methods are used in semen analysis, light microscopy is still used for routine morphological evaluation. The most used of the methods is Papanicolaou staining. This method is routinely used in Andrology laboratory for Human sperm morphology staining. In some domestic animals such as bull, R...

Preliminary treatment of urinary proteins improves electrophoretic analysis and immunodetection.

Analysis of urinary protein composition is an important tool in studies on renal physiology and physiopathology. Urine is, however, a complex mixture containing, besides protein, a variety of compounds such as salts, peptides, oligosaccharides, and glycosaminoglycans. Some of these compounds interfere with the electrophoretic migration of protein in sodium dodecyl sulfate-polyacrylamide gels an...

SDS-PAGE analysis of urinary proteins in dogs with heartworm disease

The aim of the study was to describe the urinary electrophoretic pattern of dogs with heartworm disease.Urine samples from 15 heartworm-infected and 15 healthy dogs were taken. Urinary specific gravity, urinaryprotein concentration and the urine protein/creatinine (U P/C) ratio were determined. Urine proteins werefractionated using SDS-PAGE. Results showed statistically significant differences ...

Potential Urinary Biomarkers of Acute Kidney Injury in Domestic Animals